In all other hematopoietic lineages and stages analyzed, EGFP expression was also increased, but to a lesser extent (1.3 times to 1.7 times, with a P value of 0.03 in T cells, 0.065 in Ter119+, and P > 0.1 in all other subpopulations, according to t test) (Figure 3b). Moreover, the interanimal variability in EGFP marking and expression levels was consistently reduced when using the high MOI for transduction of repopulating cells (Table 1; Figure 3a, b). As the increase in EGFP expression was strongest in CD11b+ cells and similar to that observed in fibroblasts with an elevation of the MOI from 1 to 5 (Figure 2b), at least a subset of CD11b+ cells may have originated from a subpopulation of transplantable progenitor cells that is highly susceptible to retroviral transduction (see Discussion)..
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